| Autor: |
Di MA, Chao CHEN, Wei WU, Yan YOU, Yi-yun PAN, Meng-ting SHENG, Feng ZHU, Zhi HE |
| Jazyk: |
čínština |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 38, Iss 1, Pp 15-18 (2013) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To investigate the neuroprotective effect and its mechanism of n-butanol extract (n-BuE) of Chinese toon seeds on mouse with ischemia-reperfusion (I-R) injury of the brain in vivo. Methods The brain I-R model was established in 42 mice by ligation of bilateral common carotid arteries for 2 times combined with injection of sodium nitroprusside. Animals were randomly divided into 6 groups (7 each): sham-operated group, I-R model group, vehicle control group, two treatment groups [treated with n-BuE of Chinese toon seeds 28mg/(kg.d) or 42mg/(kg.d), respectirely, for 7 days], and positive control group [treated with aspirin 14mg/(kg. d)]. The animals were sacrificed by decapitation after the experimental procedure, and the water content of brain tissue was determined by weighing. The permeability of the blood brain barrier was evaluated by measurement of Evans blue (EB) content with spectrophotometer. The contents of malondialdehyde (MDA) and nitrogen mono-oxide (NO), and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in brain tissue were determined with spectrophotometer. Results The water content and EB content of brain tissue, and the contents of MDA and NO, and the activities of SOD and GPX in hippocampal tissue increased significantly in I-R model group than in sham-operation group (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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