Detection of a BSA-Biotin-Conjugate by a Novel Immunosensor
| Autor: | Lok Hang MAK, Meinhard KNOLL, Nico DANKBAR, Tanja FISBECK, Andreas GORSCHLÜTER |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Sensors & Transducers, Vol 113, Iss 2, Pp 140-149 (2010) |
| ISSN: | 1726-5479 2306-8515 |
| Popis: | We describe a new kind of biosensor for the electro-magnetic sensing of analytes such as antibodies. In the present study the molecules bovine serum albumin (BSA) and the corresponding antibody (Anti-BSA) as well as biotin and NeutrAvidin are used as model systems. The analyte molecules are labelled with superparamagnetic microbeads and detected by sensor transducers with biofunctionalised microelectrodes. It is shown that a carboxyl-modified dextran (CMD) layer is well suited as an immobilization matrix for ligands on the microelectrode surface. The CMD layer offers a suitable and stable surface for binding of the antibodies and also suppresses unspecific binding of protein/antibody coated microbeads to the electroactive platinum surface. |
| Databáze: | OpenAIRE |
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