| Autor: |
Xiao XU, Xu-yi ZHANG, Jun-ling YAN, Chong CHEN, Xin ZHAO, Ming-hui LI, Su-yang TANG |
| Jazyk: |
čínština |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 41, Iss 12, Pp 992-997 (2017) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To explore the possibility of establishing the human adipose-derived mesenchymal stem cells (hADSCs ) bank as to provide an alternative source for the seed cells of tissue engineering. Methods The cell surface antigens of the purified, expanded hADSCs and the ones following cryopreservation were detected by flow cytometry, cultured in an induced culture medium to induce the osteogenic and adipogenic differentiation. The specific marker alkaline phosphatase (ALP) in osteogenic special medium was also detected by immunohistochemistry. Results The phenotype and expansion possibility of hADSCs after cryopreservation were remained. It could expand for 10 generations. The doubling time was 48 hours. The 2nd, 6th and 10th generation of hADSCs kept stronger ability of osteogenic and adipogenic differentiation. Conclusion The bank of hADSCs has been incipiently established and can provide eligible seed cells for tissue engineering. DOI: 10.11855/j.issn.0577-7402.2016.12.05 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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