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Objective To observe the effect of Tenghuanglin (THL) on peripheral T and B lymphocyte subsets in microwave irradiated rats, and explore the protective effect of THL on injury to immunity induced by microwave irradiation and its mechanism. Methods Eighty healthy male SD rats were randomly divided into 4 groups (n=20): normal control group (CON), radiation group (RAD), radiation + Anduolin (positive control drug) treatment group (ADL), and radiation + THL treatment group (THL). Rats in ADL and THL groups received gavage of the individual drug once a day for 7 days, and then exposed to 30mW/cm2 microwave whole body for 15min. Rats in RAD group received same dose of microwave irradiation, and those in CON group received sham irradiation. The animals were sacrificed on 7th and 14th day after irradiation, and the changes in peripheral blood smear was observed, and CD3+, CD4+ and CD8+ T lymphocyte subsets, and CD45RA+ B lymphocyte subset were determined. Results Fourteen days after irradiation, peripheral red blood cells in RAD group was obviously lower than those in CON, ADL and THL groups, and there was no statistically significant difference of red cell count between CON, ADL and THL groups. Seven days after irradiation, peripheral CD3+, CD4+ and CD8+ T cells in RAD group were lower in number than those in CON group, and they did not return to normal level up to 14th day. The peripheral CD3+, CD4+ and CD8+ T cells in THL and ADL groups were obviously higher than those in RAD group, and peripheral CD3+, CD4+ T cells were still higher than those in RAD group 14d after irradiation, while the peripheral CD3+, CD4+ and CD8+ T cells in THL and ADL groups were similar both at 7th and 14th day. Seven days after irradiation, the CD4/CD8 ratio was lower in RAD group than that in CON and THL groups (P |
