| Autor: |
Batuk DABHI, Kalpen CHAVDA, Anand JINDANI, Vishwa DHINOJA, Madhavi PATEL, Hetal JEBALIYA, Yashwantsinh JADEJA, Denish KARIA, Anamik SHAH |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Turkish Journal of Pharmaceutical Sciences, Vol 10, Iss 3, Pp 367-376 (2013) |
| ISSN: |
2148-6247 |
| Popis: |
A reliable and sensitive isocratic stability indicating RP-UPLC method has been developed and validated for quanti-tative analysis and content uniformity study of Amisulpride in tablets. An isocratic method for analysis of Ami-sulpride was achieved on C18 (100x2.1) mm particle size 1.7 µ columns at of 0.20 mL/min flow rate within shorter runtime of 3 min. Photodiode array detector was used to monitor the eluate at 280 nm. The mobile phase consis-ted of Buffer-ACN (50:50 v/v), (Buffer: 2 mL Ortho phosphoric acid in 1L water). The drug was subjected to oxida-tion, hydrolysis, photolysis and thermal degradation. Response was a linear function of drug concentration in the range of 20-80 µg/ml (r2= 0.999) with a limit of detection and quantification of 0. 1 and 0.3 µg/mL respectively. Ac-curacy (recovery) was between 99 to 101 %. Degradation products resulting from the stress studies did not interfe-re with the detection of Amisulpride the assay is stability-indicating |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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