| Popis: |
Objective To observe the effects of intrathecal injection of fluorocitrate, a glial metabolism inhibitor, on bee-toxin-induced spontaneous pain, hyperalgesia and inflammatory response. Methods Forty adult male SD rats with intrathecal catheterization were randomly divided into five groups (8 each): (1) bee-toxin alone group; (2) vehicle (solvent) group; (3) low dose (1nmol) fluorocitrate group; (4) middle dose (10nmol) fluorocitrate group; (5) high dose (50nmol) fluorocitrate group. After the measurement of rat paw withdrawal mechanical threshold (PWMT) and paw volume (PV), the drug or vehicle was administered intrathecally. Twenty minutes later, bee-toxin (0.2mg/50μl) was intraplantarly injected into the left hind paw of every rat, and spontaneous flinching reflexes (SFR) were observed instantly for 1 hour. Two hours later, PWMT and PV were measured again. Results Intraplantar injection of bee-toxin into one hind paw of rat induced persistent SFR lasting for 1 hour, with PWMT decreased and PV increased in the injected paw. Compared with control group, pretreatment with intrathecal injection of fluorocitrate produced a significant inhibition of bee-toxin-induced persistent SFR (P < 0.05, P < 0.01), decreased the PWMT in a dose-dependent manner (P < 0.05), but it had no effect on bee-toxin-induced paw edema. Conclusion Activation of spinal glial cells may participate in bee-toxin-induced spontaneous pain and mechanical hyperalgesia, but not inflammatory response. |
