| Popis: |
An experiment was carried out to evaluate the addition of proline, carnitine in Tris-extender on the viability of bull sperm following storage at 5oC. Semen was collected by means of artificial vagina (AV), diluted in Tris-extender containing 5% V/V egg yolk (EY) and 4% V/V glycerol to get a final concentration of 50 million sperms/ml. Diluted semen cooled to 5oC for 45 minute and stored at those temperature for 1, 3, 10, and 13 days. In the first activity the addition of 15, 30 and 60 mM carnitine in Tris-extender while in the second activity the inclusion of 15, 30, and 60 mM proline on the viability of sperm was investigated. Addition of carnitine to Tris-extender signifinatly increase (P |
