Spectrophotometric and Refractometric Determination of Total Protein in Avian Plasma
| Autor: | Rodica Căpriță, Adrian Caprita, Iuliana Cretescu, Valeria Nicu |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Scientific Papers Animal Science and Biotechnologies, Vol 46, Iss 2, Pp 162-165 (2013) |
| ISSN: | 2344-4576 1841-9364 |
| Popis: | The aim of this study was to compare the total protein values obtained in heparin plasma of chickens by a spectrophotometric technique (biuret method), and the values obtained on the same day in the same samples by refractometry. The results obtained by refractometry (average value 2.638±0.153g%) were higher than those obtained by the spectrophotometric method (average value 2.441±0.181g%). There was a low correlation (r = 0.6709) between the total protein values, determined with both methods. Protein is the major determinant of plasma refractive index, but glucose contributes too. The refractometric method is not recommended in chickens for the determination of total protein, because avian blood glucose concentration averages about twice than in mammalian blood. |
| Databáze: | OpenAIRE |
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