| Autor: |
Shang-Qing Huang, Yi Wen, Bing Wang, Shuai Li, Xiao-Hui Yuan, Hong-Yu Sun, Li-Jun Tang |
| Jazyk: |
čínština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 45, Iss 12, Pp 1215-1221 (2020) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To investigate the potential mechanism of abdominal paracentesis drainage (APD) on severe acute pancreatitis (SAP) through regulating Toll-like receptor 4 (TLR4) expression in rat intestine. Methods Male SD rats were randomized into the Sham group, SAP group, and APD group, with 15 rats in each group. Rats were treated with a retrograde injection of 5% sodium taurocholate into the pancreatic bile duct to induce SAP, followed by ADP treatment through placing a drainage tube in the right lower abdomen of rats. Rats without ADP treatment were used as controls: SAP group included rats without ADP surgical procedure; while Sham group included rats with ADP surgical procedure but without placing a drainage tube. About 24 hours after ADP treatment, pathological changes of small intestine and pancreas were observed by HE staining; serum amylase and lipase activities were detected by the automatic biochemical analyzer; serum endotoxin levels were detected by limulus amebocyte lysate method; serum DAO activity was detected by ultraviolet colorimetry; TNF-α, IL-6 and D-lactic acid levels were detected by ELISA; apoptosis of intestinal mucosal epithelial cells was detected by TUNEL assay; mRNA expression of TLR4 was detected by real-time PCR; the expression of TLR4 signaling pathway and apoptosis-related proteins were detected by immunohistochemistry and Western blotting. Results Compared with SAP rats, the pathological damage of small intestine and pancreas of APD rats was significantly reduced; the pathological score was decreased (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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