Premilinary studies for optimiziing a protocol for obtaining embryogenic calluses in two rubber (Hevea brasiliensis Mull. Arg) clones from different geographical origins

Autor: Santiago Cadavid Ruiz, César Augusto Hernández Rendóri, Rodrigo Hoyos, Marisol Medina S., Luis Fernando Restrepo
Jazyk: Spanish; Castilian
Rok vydání: 2007
Předmět:
Zdroj: Revista Colombiana de Biotecnología, Vol 8, Iss 1, Pp 32-47 (2007)
ISSN: 1909-8758
0123-3475
Popis: The influence of growth regulators on obtaining friable rubber (Hevea brasiliensis Müll. Arg.) calluses with no plant regeneration as investigated. Two clones having different geographical origin were used in all trails carried out in this study: FX 3864 (South-American) and PB 254 (Asian). Young leaves and eight- to ten-week-old seed integument from both clones were used as explants in several experiments; they were initially cultured in MH medium (Carron, et ál., 1989), modified MH medium (Montoro, et ál., 1993, 2000) and modified MS medium (Carron, et at,. 1992), no positive response being obtained by days 25 or 50. However, other trials were carried out with the integument in modified MS medium (1962), 0.67 mg/L BAP and 0.66 mg/L 2-4 D being added as medium for initiating embryogenesis, the formation of white, friable calluses being observed by day 25 in the two selected clones. These calluses were sub-cultured in MS supplemented with 0.35 mg/L BAP and 0.2 mg/L 2-4 D as callogenesis expression medium, embryogenic expression being observed in both clones by day 50. Equally friable white calluses were obtained from young leaves in the two clones in MS medium supplemented with 1.0 mg/L BAP, 1.0 mg/L ANA but without IBA and kinetin by day 25. Calluses sub-cultured in the same medium supplemented with 0.5 mg/L BAP and 0.5 mg/L ANA began to show increased friability after 50 days. culture. This work is a partial report of a macro-project for optimising a protocol for rubber (Hevea brasiliensis) multiplication by somatic embryogenesis.
Databáze: OpenAIRE
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