| Autor: |
Hong KUANG, Yuan ZHANG, Cong ZHANG, Shu-fang JIA, Yi SUN, Jing LI, Yong-he HU |
| Jazyk: |
čínština |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 37, Iss 12, Pp 1115-1119 (2012) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To prepare a novel biomimetic agent with adhesive and osteoinduction capabilities, and provide a new approach for improving seeding cell adhesion and osteoblastic differentiation on a scaffold surface. Methods A novel bifunctional recombinant protein was engineered and prepared by bioinformatics, standard molecular cloning, protein expression and purification techniques. A centrifugal cell adhesive assay and an osteoinduction assay were employed to determine the biofunction ex vivo. Results A 1954bp long DNA chimera of FN Ⅲ7-10/CAD11 EC1-2 and recombinant FN Ⅲ7-10/CAD11 EC1-2 of 641 amino acids were obtained. Polystyrene surface coated with this protein significantly increased the adhesive property and osteodifferentiation of human bone marrow mesenchymal stem cells. Conclusion rFN/CAD possesses dual biofunctions of osteoblastic adhesion and differentiation, which can be further applied as an ideal biomimetic molecule for surface modification. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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