MOLECULAR CHARACTERISATION OF CHLAMYDOPHILA PSITTAC/ ISOLATES BY RESTRICTION ENDONUCLEASE ANALYSIS OF DNA
| Autor: | Sreeja R. Nair, M. Mini, V. Jayaprakasan, G. Krishnan Nair |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Journal of Veterinary and Animal Sciences, Vol 40, Iss 1, Pp 1-5 (2009) |
| ISSN: | 2582-0605 0971-0701 |
| Popis: | Reports on the prevalence of abortion due to Chlamydia psittaci (Chlamydophila abortus) in Kerala have indicated a need for the in depth study of fhe organism. To unearth differences among fhe isolates of Chlamydia psittaci, at molecular level, restriction enzyme analysis of genomic DNA and plasmid profiling were carried out. Four isolates from four different sources were used in this study. DNA extracted from these isolates were digested by Eco Rl, Hae 11! and Bam Hl. Hind 111 digested Lambda DNA was used as molecular weight marker. The isolates were screened for the presence of plasmids also. Characterisation of chromosomal DNA of Chlamydophila abortus by restriction enzyme analysis revealed a near homogeneity among the isolates. The lack of plasmids in al! isolates also indicates the homogeneity of their origin and probably the genetic relationship. Thus, the restriction enzyme digestion analysis with other genetic tools can better resolve the similarity or dissimilarity among the isolates |
| Databáze: | OpenAIRE |
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