Genetic diversity and population structure of Vibrio choierae in Colombia
| Autor: | Diego Mauricio Riaño Pachón, Emilia María Valenzuela de Silva, José Ramón Mantilla Anayá, Carlos Agudelo |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Revista Colombiana de Biotecnología, Vol 5, Iss 1, Pp 36-44 (2007) |
| ISSN: | 1909-8758 0123-3475 |
| Popis: | Pulsed field gel electrophoresis (PFGE) was used to study thirty-four Vibrio choierae isolates from the Colombian cholera epidemic between 1991 and 1996. Three Vchoierae isolates not belonging to the epidemic in Colombia and a Vfluvialis isolate were also analysed. The genetic diversity observed for the 38 isolates typified was 0.95, a very similar valué to that obtained by Multilocus Enzyme Electrophoresis (MLEE). No cor reía tion was observed between the variables characterising these isolates (serotype, origin [clinical or environmental], state, electrophoretic type (MLEE) and restriction macro-fragment profile or pulsetype (PFGE)). No evidence of linkage disequilibrium was obtained on evaluating this population with PFGE and MLEE genetic markers. It is suggested that the Vibrio choierae population in Colombia presents a sexual genetic structure. The lack of evidence for linkage disequilibrium and the absence of correlation between the epidemiological, biochemical and molecular variables available sustains such a hypothesis. It is also sustained by the high valué for genetic diversity obtained which could be reflecting the coexistence of several lines of descent having a high genetic flow amongst them. Key words: Vibrio choierae; Pulsed-Field Gel Electrophoresis; molecular epidemiology; population genetics; linkage disequilibrium. |
| Databáze: | OpenAIRE |
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