| Autor: |
Dong-Hai Liu, Tian-Ning Zhao, Jia-Qi Yang, Xue-Tong Tao, Jing-Bo Zhang, Yu-Ran Chen, Lu-Lu Yang, Xue Liu, Hai-Peng Song, Jin-Ling Zhu |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Journal of Hainan Medical University, Vol 25, Iss 10, Pp 23-27 (2019) |
| ISSN: |
1007-1237 |
| Popis: |
Objective: To investigate the expression of miRNA21 in hippocampus of rats with pentylenetetrazol-induced epilepsy and its possible mechanism. Methods: Twenty-four healthy male wister rats was randomly divided into two groups, the normal group and the epilepsy group. The Racine grade scores of the two groups of rats were recorded to evaluate the establishment of a rat model of epilepsy. The differentially expressed of microRNAs targeting bcl-2 in the hippocampus of epileptic rats was screened by bioinformatics method. The expression of microRNA21 in hippocampus of epileptic rats was verified by qRT-PCR. Western blot and immunohistochemistry were used to detect the expression of Bcl-2 in rat hippocampus; Hoechst 33258 was used to detect the apoptosis of rat hippocampal neurons. Results: The Racine score of the rats in the epilepsy group was significantly higher than that in the normal control group (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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