| Autor: |
Lei JI, Qiang CHEN, Yu-qin HE, Ling LIN, Ya-mei RAN, Xiu-qiong LANG, Lei LI, Jian-ming WANG, Zheng-guo WANG, Min YANG |
| Jazyk: |
čínština |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 39, Iss 10, Pp 771-775 (2014) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To explore the potential role of HCN2-GLT-1 [hyperpolarization-activated cyclic nucleotide-gated (HCN)-2 channel and astrocytic glutamate transporter 1 (GLT-1)] in co-mediating visceral hyperalgesia at the spinal cord level following exposure to PTSD (posttraumatic stress disorder)-like stress in rats. Methods Adult female SD rats were randomly divided into normal control group (n=14), PTSD group (n=15) and PTSD+CTX group [pretreated with ceftriaxone (CTX), n=15]. The animal model of PTSD was reproduced by using single-prolonged stress (SPS) combined with electric shock. The alteration of visceral sensitivity was evaluated by measuring the viscero-motor response (VMR) to graded colorectal distension (CRD) 7 days after PTSD. The transcription of the gene encoding the GLT-1 was selectively induced by CTX to up-regulate the GLT-1 expression. The expression of spinal HCN2 and the effect of CTX on it were evaluated by immunofluorescence. Results HCN2 expression increased significantly in the spinal cord within 7 days after the exposure to PTSD as compared with that of control group (78.05±6.49 vs 121.12±4.85, P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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