| Autor: |
Mayyan Mubarak, Nayef Al Gharaibeh, Samuel Sommaruga, Jie Li, Alexander Vortmeyer |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Journal of Kidney Cancer and VHL, Vol 8, Iss 3 (2021) |
| ISSN: |
2203-5826 |
| Popis: |
Using a novel 3dimensional approach, we tracked histological changes to elucidate earliest stages of renal clear cell neoplasia in normal kidney tissue of patients with VHL disease. Tissue blocks of interest were procured, followed by serial sectioning and 3-dimensional reconstruction of the entirety of pathologic events. The results reveal an abundance of foci with aberrant clear cell proliferations that initially develop along the tubular lining, but have the potential to aggregate within individual tubules. This stage is followed by extension of clear cell aggregates beyond the tubular basement membrane which allows for recruitment of angiogenesis derived from interstitial vasculature. The results suggest that the most frequent pathologic event in VHL kidneys is the presence of isolated or aggregated clear cells within the tubular epithelium, potentially developing further into a protracted process of neoplasia. The abundance of independent pathologic events in VHL kidneys confirms developmental mechanisms to precede tumor initiation.To our knowledge, this is the first report demonstrating that tracking of histologic changes in the 3rd dimension enables the confirmation of the sequence of events from the earliest pathologic change in VHL kidney to the neoplastic stage. This approach is not only useful for visualization and quantification of pathologic changes, but also for targeted sampling allowing selective analysis of the earliest stages of clear cell carcinogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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