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Objective: To investigate the inhibition effect of dexamethasone with different concentration gradients on the expression of MMP-1, -3, and -13 in a bone joint cell model of IL-1β-induced SW1353 cells. Methods: The SW1353 cells were placed in a culture medium containing 101-108 nmol/L dexamethasone, and after 12 hours of culture, the appropriate intervention concentration range of dexamethasone was screened by the MTS for subsequent experiments. After SW1353 cells were induced with 10 ng/mL IL-1β, 101-107 nmol/L dexamethasone was given for intervention, and RT-PCR was used to detect the mRNA expression of MMP-1, -3, and -13. The protein was extracted after the intervention of 104 nmol/L dexamethasone, and western blot was used to detect the protein expression of MMP-1, -3, and -13. Results: MTS proliferation experiment results showed that 101-107 nmol/L dexamethasone had no significant effect on SW1353 cell viability, while 108 nmol/L dexamethasone had a significant inhibitory effect on SW1353 (p |
