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In this study, the identification and genetic analysis of a stable genetic low-temperature-sensitive leaf color mutant tcd32 obtained by 60Co gamma mutagenesis in the japonica rice (Oryza sativa L.)"Jiahua 1" were carried out. Under the condition of 20℃, the tcd32 mutant showed white leaves and obviously low level of photosynthetic pigments, have not intact chloroplast, eventually mutant plants withered to death. And under the condition of 25℃, the 3-leaf seedlings were yellow, then began to turn yellowish green from the fourth leaves, which also showed lower level of photosynthetic pigments. However, at 30℃, the tcd32 mutant had no obvious difference from the wild type phenotype. Based on genetic analysis of F2 segregating population form the cross of Peiai 64S and tcd32, it was shown that the low-temperature-sensitive mutant trait was controlled by a single recessive gene (tcd32). Then through map-based cloning, the TCD32 gene was located to the 537 kb region at the top of chromosome 3, which is a novel gene associated with early chloroplast development in rice. |
