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Objective To observe the changes in oxygen metabolism and sonic hedgehog (SHH) signaling pathway in soldiers returning to plains after being stationed and trained for 6 months in a plateau. Methods Eighty male officers and soldiers, aged 20-30 (22.3±2.9) years, after being stationed and trained on plateau (altitude 3960m) for 6 months and returned to plain region (altitude 200m), were selected as subjects. Before their returning to plateau, 6 months after their station and training in plateau, and 2 days after their returning to plain, fasting venous blood samples were collected, the serum levels of superoxide dismutase (SOD), malondialdehyde (MDA) and Sonic Hedgehog (SHH) were determined by ELISA, the transcription of SHH mRNA was assayed by RT-PCR, and the expressions of SMO and nucleoprotein GLI2 were detected by Western blotting. All the data mentioned above were collected for statistical analysis. Results As the subjects entered and garrisoned in plateau for 6 months, the activity of SOD decreased and the content of MDA increased significantly (P < 0.05). Both the protein expression and mRNA transcription of SHH were significantly higher after staying in plateau than in plain. When they returned to plain, both parameters decreased significantly, but were still higher than that when they lived in plain (P < 0.01). The expressions of SMO and nucleoprotein GLI2 showed a same tendency of changes. Conclusion High altitude environment may have a great influence on oxygen metabolism of organism and SHH signal pathway, and the hypoxic environment of high altitude region is one of the conditions in activating the SHH signal pathway. |
