| Autor: |
Li-mei WU, Yuan-yuan HUA, Yu-tong WU, Chen-guo YAO, Rui-zhe ZHANG, Zhi-liang WANG, Zheng-ai XIONG |
| Jazyk: |
čínština |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Medical Journal of Chinese People's Liberation Army, Vol 40, Iss 9, Pp 699-703 (2015) |
| ISSN: |
0577-7402 |
| Popis: |
Objective To investigate the influence of picosecond pulse electric field (psPEF) to the invasive ability of cervical cancer. Methods The model of cervical cancer was reproduced in BALB/c nude mice (n=24), and they were randomly divided into four groups (n=6) when the xenografts had grown reaching a diameter of 0.8-1.0cm: control group (psPEF was not given), low field intensity group (50kV/cm), moderate field intensity group (60kV/cm) and high field intensity group (70kV/cm). Seven days after the psPEF treatment, the histomorphological changes were observed with HE staining and transmission electron microscopy (TEM), the expressions of vascular endothelial growth factor (VEGF) and matrix metalloproteinases-9 (MMP-9) were determined with immunohistochemical (IHC) staining, and the changes in protein level of VEGF and MMP-9 were assessed with Western blotting. Results After psPEF treatment, the area of necrosis was found to be increased with an increase in psPEF intensity. With TEM different degrees of apoptosis and necrosis in tumor cells with an increase of psPEF intensity were found. IHC showed that the number of VEGF and MMP-9 positive cells in cancer tissue was decreased with an increase in psPEF intensity. The average optical density (AOD) of VEGF and MMP-9 proteins decreased significantly in psPEF treatment groups compared with that in control group, and the AOD values in psPEF treatment groups decreased with an increase in psPEF intensity, and the decrease was statistically significant (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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