Prevalence of sea, seb, tsst, and mecA Genes in Staphylococcus aureus Isolated from Shrimps Sold in Seafood Retailers in Tehran, Iran

Autor: M.M. Soltan Dallal, R. Mazaheri Nezhad Fard, M.K. Sharifi-Yazdi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Food Quality and Hazards Control, Vol 5, Iss 2, Pp 72-76 (2018)
ISSN: 2345-6825
Popis: Background: Staphylococcus aureus, a Gram-positive bacterium, is the most prevalent food-borne pathogen in most regions of the world. The current study was carried out with the aim of S. aureus isolation from shrimps sold in Tehran, Iran. Furthermore, the genes of mecA as indicator of methicillin-resistant S. aureus, sea, seb, and tsst encoding enterotoxins were studied in the S. aureus isolates. Methods: Totally, 150 fresh and 150 frozen shrimp samples were collected from seafood retailers in Tehran. Isolation of S. aureus from the samples was carried out using conventional methods. The target genes were identified using polymerase chain reaction technique. Data were statistically analyzed using SPSS v. 11.5 software. Results: Out of 150 fresh and 150 frozen samples, 84 samples (28%) were contaminated with S. aureus. The contamination rate in fresh samples (22%) was significantly (p
Databáze: OpenAIRE