Influence of cortical nanoparticles on the embryonic development of three seabirds: Arbacia lixula, Paracentrotus lividus and Sphaerechinus granularis
| Autor: | Alač, Iva |
|---|---|
| Přispěvatelé: | Lyons, Daniel, Burić, Petra |
| Jazyk: | chorvatština |
| Rok vydání: | 2018 |
| Předmět: |
uspješnost oplodnje
Sphaerechinus granularis Paracentrotus lividus PRIRODNE ZNANOSTI. Interdisciplinarne prirodne znanosti. Znanost o moru fertilization success cerium (IV) oxide nanoparticles tretman sperme Arbacia lixula nanočestice cerijevog (IV) oksida SUEDT sperm treatment NATURAL SCIENCES. Interdisciplinary Natural Sciences. Marine Science |
| Popis: | Nanočestice cerijevog (IV) oksida (NP CeO2) imaju široku primjenu uključujući funkcionalne premaze, elektroniku, tehniku, medicinu te su prisutne u okolišu kao sekundarni produkt motora s unutarnjim izgaranjem (dodatak gorivu). Posljednje spomenuto može biti od posebne važnosti, jer se očekuje da će opterećenje okoliša proizašlo iz ispušnih plinova automobila biti značajno. Cilj ovog rada bio je ispitati potencijalnu toksičnost NP CeO2 na tri vrste ježinaca: Arbacia lixula, Paracentrotus lividus i Sphaerechinus granularis primjenom testa embrionalnog razvoja ježinca (SUDET). Nakon izlaganja zigota koncentracijama NP CeO2 u rasponu od 10 - 10 000 μg/L, embrionalni razvoj sve tri vrste se nastavio bez značajnih razlika u odnosu na kontrolni uzorak. Sperma ježinaca tretirana je s NP CeO2 u koncentracijskom rasponu od 10 - 10 000 μg/L. Rezultati su pokazali da NP CeO2 nisu imale negativan učinak na spermu. Naime, više od 90% jajnih stanica kod sve tri vrste bilo je uspješno oplođeno. Nadalje, tako oplođene jajne stanice uspješno su se razvile do stadija morfološki normalne larve pri svim koncentracijama, osim pri koncentraciji od 10 000 μg/L. Rezultati su pokazali da NP CeO2 kada su tretirane larve vrsta A. lixula i P. lividus nakon 48 h pokazale samo 74.25 ± 8.42% i 82.25 ± 2.63% normalno razvijenih larvi. S toga, testom embrionalnog razvoja ježinaca nije dokaznao da su ispitivane NCeO2 materijal zbog kojeg bi se trebalo brinuti, niti pri visokim koncentracijama. Međutim, potrebno je provesti dodatna istraživanja prije nego se zabrinutost oko nanočestica CeO2 može u potpunosti otkloniti. Cerium (IV) oxide nanoparticles (NP CeO2) have a wide variety of applications including as a component in functional coating, in electronics, engineering, medicine, and are present in the environment as secondary products of combustion engines due to their use as fuel additives. It is the latter which may be of particular concern as the environmental load deriving from automobile exhaust is expected over the coming years to be significant. The aim of this work was to investigate the potential embryotoxicity of NP CeO2 on three sea urchin species: Arbacia lixula, Paracentrotus lividus and Sphaerechinus granularis using the sea urchin embryo development test (SUEDT). Embryonal development following the exposure of zygotes to NP CeO2 at concentrations ranging between 10 - 10000 μg/L proceeded for all three species without significant differences compared to the control. Sea urchin sperm was treated with NP CeO2 at the same concentrations as for the zygotes and results indicated that NP CeO2 did not affect the fertilization ability of sea urchin sperm, with more than 90% of eggs for each species successfully fertilized. These eggs developed into morphologically normal larvae at all NP concentrations except for the 10000 μg/L NP CeO2 treatment where larvae of A. lixula and P. lividus showed only 74.25 ± 8.42% and 82.25 ± 2.63% normally developed larvae after 48h, respectively. Thus, the sea urchin embryo development test did not indicate that the investigated NP CeO2 is a material of concern, even at high concentrations. However, a broader range of 57 bioassays must be conducted before concerns about the potential toxicity of cerium oxide nanoparticles may be completely alleviated. |
| Databáze: | OpenAIRE |
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