Antiproliferative effect of Pterogyne nitens on melanoma cells
| Autor: | Regasini, L. O., Lopes, A. A., Dulce Silva, Furlan, M., Young, M. C. M., Maria, D. A., Barreiro, E. J., Bolzani, V. S. |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Instituto de Botânica, São Paulo, Instituto Butantan, Universidade Federal do Rio de Janeiro (UFRJ) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Farmacologia
Caesalpinioideae Fabaceae Ciências Farmacêuticas Atenção Farmacêutica Plantas medicinais lcsh:RS1-441 Farmácia Indústria Farmacêutica antiproliferative pterogyne nitens caesalpinioideae melanoma flavonoids fabaceae lcsh:Pharmacy and materia medica Botânica médica lcsh:HD9665-9675 Assistência farmacêutica lcsh:Pharmaceutical industry Pterogyne nitens Toxicologia Melanona |
| Zdroj: | Revista de Ciências Farmacêuticas Básica e Aplicada, Vol 28, Iss 3 (2007) Revista de Ciências Farmacêuticas Básica e Aplicada, Vol 28, Iss 3, Pp 335-340 (2009) Revista de Ciências Farmacêuticas Básica e Aplicada Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP ResearcherID |
| ISSN: | 1808-4532 |
| Popis: | Made available in DSpace on 2014-06-24T19:53:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2007Bitstream added on 2014-06-25T11:46:44Z : No. of bitstreams: 1 ISSN1808-4532-2007-28-3-335-340.pdf: 174029 bytes, checksum: 4ac1489d6219f7ab068475695abbee5b (MD5) As part of our program of bioprospecting for novel antitumor drug prototypes, twenty extracts and fractions obtained from Pterogyne nitens Tul. (Fabaceae, Caesalpinioideae) were screened for antiproliferative activity against B16F10 murine melanoma cells, by the MTT colorimetric assay. The strongest activity was found in EtOAc fractions from the flowers (IC50 = 0.35 µg/mL), fruits (IC50 = 0.34 µg/mL), leaves (IC50 = 0.33 µg/mL) and stems (IC50 = 0.29 µg/mL). Analysis by TLC and HPLC-DAD showed the presence of guanidine alkaloids, flavones and flavonols in the bioactive samples. Additionally, a phytochemical study of the EtOAc fraction of the stems afforded quercetin (1) and isoquercitrin (2), two flavonols with antiproliferative activity previously described in the literature. On the basis of these results, it can be concluded that P. nitens inhibits the growth of melanoma cells in vitro. Further investigations will be needed to assess the usefulness of the samples under study for the treatment of neoplasms and to characterize other bioactive compounds. Keywords: antiproliferative; Pterogyne nitens; Caesalpinioideae; melanoma; flavonoids; Fabaceae. Departamento de Química Orgânica, NuBBE, Instituto de Química, Universidade Estadual Paulista, UNESP, Araraquara, SP, Brasil. Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, SP, Brasil. Serviço de Bioquímica, Divisão de Ciências Fisiológicas e Químicas, Instituto Butantan, São Paulo, SP, Brasil. Faculdade de Farmácia, LASSBio, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, RJ, Brasil. Departamento de Química Orgânica, NuBBE, Instituto de Química, Universidade Estadual Paulista, UNESP, Araraquara, SP, Brasil. |
| Databáze: | OpenAIRE |
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