ANGIOGENEZA I LIMFANGIOGENEZA U KARCINOMU ENDOMETRIJA I NJIHOV PROGNOSTIČKI ZNAČAJ
| Autor: | Vidović, Anja |
|---|---|
| Přispěvatelé: | Babarović, Emina, Mustać, Elvira, Matušan Ilijaš, Koviljka, Finderle, Aleks |
| Jazyk: | chorvatština |
| Rok vydání: | 2019 |
| Předmět: | |
| Popis: | Karcinom endometrija četvrta je najčešća zloćudna bolest žena u RH, a incidencija javljanja u stalnom je porastu. Prognostički čimbenici za ovu bolest su brojni, a njihovo razumijevanje ograničeno. Cilj ovog istraživanja bio je analizirati angiogenezu i limfangiogenezu u karcinomu endometrija kroz utvrđivanje srednje gustoće malih krvnih žila (MVD) i limfnih žila (LVD) u centru tumora (CT) i na invazivnoj fronti (IF) te odrediti njihov prognostički značaj usporedbom s drugim poznatim prognostičkim čimbenicima. Analiziran je tumorski materijal 74 pacijentice s dijagnozom karcinoma endometrija koje su u periodu od 2002. do 2006. godine liječene na KBC Rijeka. Medijan dobi sudionica iznosio je 66 godina s rasponom od 33 do 89 godina. Vizualizacija krvnih žila postiguta je tretiranjem antitijelom CD 31 i kromogen crvenilom, dok su limfne žile vizualizirane antitijelom D2-40 i diaminobenzidinom. Uvidom u patohistološku i drugu medicinsku dokumentaciju, prikupljena su svi relevantni podaci za sudionice ovog istraživanja. Svjetlosnim mikroskopom evaluirana je gustoća krvnih i limfnih žila u CT i na IF tumora te prisutnost limfovaskularne invazije. Podaci su statistički obrađeni u programu MedCalc/Windows. Utvrđeno je da su MVD i LVD značajno više na IF karcinoma u odnosu na CT. MVD je značajno viša na IF u odnosu na LVD u svim promatranim skupinama tumora. U univarijantnoj analizi viši MVD CT i IF kao i prisutnost VI bili su povezani s pojavom recidiva bolesti, kraćim ukupnim preživljenjem i kraćim preživljavanjem bez povrata bolesti. VI u tumorskom tkivu karcinoma endometrija bio je nezavisni prognostički parametar pojave recidiva, ukupnog preživljenja i preživljavanja bez povrata bolesti kod bolesnica. Endometrial carcinoma is the fourth most common malignant disease in women in Croatia. Its incidence is constantly rising.Prognostic factors for this disease are numerous, but their understanding is limited. The aim of this research was to analyze angiogenesis and lymphangiogenesis in endometrial carcinoma by researching microvessel density (MVD) and lymphatic vessel density (LVD) both in the center of the tumor (CT) and on the invasive front (IF) and to determine their prognostic value through comparison with other known prognostic factors. Tumor material from 74 patients diagnosed with and treated for endometrial carcinoma between 2002. and 2006. on CHC Rijeka was analysed. Median age value was 66 with 33 as lowest and 89 as highest value. Blood vessel were visualized with CD 31 antibody and red chromogen kit, while lymphatic vessel were visualized with D2-40 antibody and diaminobenzidine. All the relevant dana was gathered by examining pathohistological and other medical documentation. MVD and LVD both in the CT and on the IF, as well as lymphovascular invasion were evaluated with optical microscope. The data was statistically analyzed with computer program MedCalc/Windows. MVD and LVD were significantly higher on the IF than in the CT. MVD, compared to LVD, is significantly higher on the IF in all tumor categories. Univariate analysis connects higher MVD CT and IF, as well as VI, with relapse of the disease, shorter overall survival and shorter relapse free survival. VI in the tumor tissue of endometrial carcinoma emerged as independent prognostic parametar of relapse occurence, overall survival and relapse free survival. |
| Databáze: | OpenAIRE |
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