Uncovering sexual dimorphism in type 2 diabetes by redox proteomics
| Autor: | Gómez-Serrano, María, Camafeita, Emilio, Vázquez, Jesús, Peral, Belén |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Fundación Pro CNIC |
| Rok vydání: | 2019 |
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| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Resumen del póster presentado al 6th Symposium on Biomedical Research: Advances and Perspectives in Molecular Endocrinology "In Homage to Gabriella Morreale", celebrado en el Instituto de Investigaciones Biomédicas Alberto Sols (IIBM-CSIC) el 31 de mayo de 2019. The prevalence of type 2 diabetes (T2DM) is rising globally due to, among other factors, obesity, physical inactivity, and aging superimposed on a genetic predisposition. Despite that the NIH recently highlighted the importance of sexual dimorphism and dictated inclusion of both sexes in clinical trials and basic research, little is known about the role of gender in adipose tissue biology. We have recently validated that antioxidant response is one of the gender-specific hallmarks of sexual dimorphism in adipose tissue. Since mitochondria is recognized as the main source of oxidative stress, in this work we aimed to better understand the impact of gender on adipocyte mitochondria by quantitating not only protein abundance changes, but also redox alterations undergone by Cys protein residues. Mitochondrial samples were obtained from adipocytes previously isolated from visceral adipose tissue biopsies collected from morbidly obese patients (BMI > 35 kg/m2) suffering T2DM who were submitted for bariatric surgery. Protein abundance and redox differences between women and men suffering T2DM were assessed following a high-throughput approach encompassing differential alkylation of reduced and reversibly oxidized Cys (FASILOX), isobaric labelling (iTRAQ) and LC-MS/MS. LC-MS/MS analysis allowed the identification of 15,763 peptides at 1% FDR, corresponding to 2,895 proteins in the mitochondrial extracts. Of note, 754 proteins were annotated in the current version of Human MitoCarta v.2 (68% coverage). FASILOX technology allowed the identification of 277 oxidized and 1990 reduced Cys-containing peptides. Cys oxidation was globally increased in diabetic men compared to women. Enrichment analyses showed that oxidation was focus on the oxidative phosphorylation (OXPHOS) system, especially in Complex I (p This work was supported by grant SAF2012-33014 from MINECO, Spain (B.P). The CNIC is supported by the Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). |
| Databáze: | OpenAIRE |
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