| Autor: |
Telwatte, Sushama, Kumar, Nitasha, Vallejo-Gracia, Albert, Kumar, Renuka, Lu, Chuanyi, Ott, Melanie, Wong, Joseph, Yukl, Steven |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
bioRxiv, vol 2, iss 01-19 |
| Popis: |
The exact mechanism of coronavirus replication and transcription is not fully understood; however, a hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-based diagnostic assays for SAR-CoV-2 are qualitative or semi-quantitative and do not provide the resolution needed to assess the complex transcription dynamics of SARS-CoV-2 over the course of infection. We developed and validated a novel panel of specially designed SARS-CoV-2 ddPCR-based assays to map the viral transcription profile. Application of these assays to clinically relevant samples will enhance our understanding of SARS-CoV-2 replication and transcription and may also inform the development of improved diagnostic tools and therapeutics. Highlights We developed a novel panel of 7 quantitative RT-ddPCRs assays for SARS-Cov-2 Our panel targets nongenic and genic regions in genomic and subgenomic RNAs All assays detect 1-10 copies and are linear over 3-4 orders of magnitude All assays correlated with the clinical Abbott SARS-CoV-2 Viral Load Assay Clinical samples showed higher copy numbers for targets at the 3’ end of the genome |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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