Electrical detection of bacteria on SOI film using the pseudo-MOSFET configuration
Autor: | Médéric Vindret, Licinius Benea, Irina Ionica, Cécile Delacour, Pascal Xavier, Jean Martins, Dominique Rauly, Eric Chamberod |
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Přispěvatelé: | XAVIER, Pascal, Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation (IMEP-LAHC ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Thermodynamique et biophysique des petits systèmes (TPS), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut des Géosciences de l’Environnement (IGE), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Recherche pour le Développement (IRD)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), IUT de Grenoble, Université Joseph Fourier - Grenoble 1 (UJF), Nanonets2Sense |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: | |
Zdroj: | HAL International Workshop on Semi-conducting Nanomaterials for Health, Environment and Security Applications International Workshop on Semi-conducting Nanomaterials for Health, Environment and Security Applications, Nanonets2Sense, Nov 2018, Grenoble, France |
Popis: | session Devices; International audience; Fast detection of bacteria is crucial in several domains such as health care, food industry and for environmental applications. Classical methods suffer from some drawbacks. For instance, traditional culture is very time consuming and PCR (polymerase chain reaction) is very expensive. For these reasons, electrical detection may be a good alternative. In our work, a Pseudo-MOSFET device realized on a SOI (Silicon on insulator) wafer was used in order to detect bacteria. To reach this goal, comparisons between transistors characteristics with and without bacteria were analyzed. E.Coli K12 strains were grown in medium (pH7) at 30°C the day before. The bacterial suspensions with a final concentration of 10 8 cells/mL were prepared in lysogeny broth (LB) medium which was then removed and replaced by distilled water in order to avoid the presence of ions from the medium. Then a drop of 5µL of this solution was put on SOI wafer. After drying, measurements were performed. A characteristic shift of the ID (VG) and the VB (VG) curves is observed thus, showing the presence of negative electric charges, on the top surface of the sensitive Pseudo-MOSFET. This negative charge could be related to the bacteria electric charges. Further experiments have to be performed to check the repeatability of the observed effect and try to enhance its magnitude. |
Databáze: | OpenAIRE |
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