Very long-chain (>C24) polyunsaturated fatty acids in marine fish: Nutritional regulation of genes involved in their biosynthesis in Sparus aurata and Solea senegalensis
| Autor: | Torres Rodríguez, M., Navarro, Juan Carlos, Monroig, Óscar, Varó, Inmaculada, Hontoria, Francisco |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Generalitat Valenciana, Navarro, Juan Carlos, Monroig, Óscar, Varó, Inmaculada, Hontoria, Francisco, Navarro, Juan Carlos [0000-0001-6976-6686], Monroig, Óscar [0000-0001-8712-0440], Varó, Inmaculada [0000-0002-3937-3846], Hontoria, Francisco [0000-0003-2466-1375] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Trabajo presentado en el VII International Symposium on Marine Sciences (ISMS), celebrado del 1 al 3 de julio de 2020, en Barcelona (España) Very long-chain (>C24) polyunsaturated fatty acids (VLC-PUFA), although present in small amounts, play important roles for the correct development and functionality of neural tissues, especially during early development of vertebrates [1]. However, studies on VLC-PUFA in fish are scarce. Their biosynthesis, mediated by elongation of very long-chain fatty acid 4 (Elovl4) proteins, is substrate-dependent. So, shorter fatty acid precursors, i. e. long-chain (C20-24) polyunsaturated fatty acids (LC-PUFA), are required. These nutrients are mostly incorporated by the diet and their bioavailability can determine the capacity of Elovl4 for satisfying the physiological VLC-PUFA demands in marine fish [2]. Thus, nutritional regulation of Elovl4, as well as other elongase and desaturase genes involved in LC-PUFA biosynthesis (Elovl5, Fads2) has been proposed as a strategy to enhance endogenous production of LC-PUFA and VLC-PUFA in fish farming [2]. This study aimed at investigating the nutritional regulation of genes involved in the biosynthesis of VLC-PUFA (elovl4a, elovl4b) and LC-PUFA (fads2, elovl5) in early life-cycle stages (larvae and post-larvae) of Sparus aurata and Solea senegalensis fed diets adapted to each development stage, live prey for larvae and microdiets for post-larvae, with a variable content in VLC-PUFA precursors, i.e. LC-PUFA. The results denoted that fads2, elovl5, elovl4a and elovl4b genes can be regulated by dietary LC-PUFA content. It is important to highlight that elovl4a and elovl4b genes, strongly expressed in neural tissues of both fish species, were differently regulated according to the species-specific VLC-PUFA putative needs associated with each early life-stage and the LC-PUFA dietary availability. These results can help to elucidate the molecular mechanism controlling the VLC-PUFA biosynthesis and their species-specific requirements along of the marine fish development. Thus, this study opens the possibility to incorporate successfully alternative lipid sources, through an early nutritional programming that stimulates the VLC-PUFA biosynthesis during the first exogenous feeding stages. This research was funded by the projects AGL 2013-40986-R (Spanish Government, Ministry of Economy and Competitiveness), and PROMETEO II / 2014/085 (Generalitat Valenciana). Miguel Torres was supported by a PhD funding program from Diputación de Castellón. |
| Databáze: | OpenAIRE |
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