P16(INK4A) immunostaining is a strong indicator for high-risk-HPV-associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low-risk-HPV-infection in head and neck papillomas and laryngeal dysplasias

Autor: Mooren, Jeroen J., Gultekin, Sibel E., Straetmans, Jos M. J. A. A., Haesevoets, Annick, Peutz-Kootstra, Carine J., Huebbers, Christian U., Dienes, Hans P., Wieland, Ulrike, Ramaekers, Frans C. S., Kremer, Bernd, Speel, Ernst-Jan M., Klussmann, Jens P.
Přispěvatelé: Ondersteunend personeel ODB, Pathologie, Moleculaire Celbiologie, KNO
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: International Journal of Cancer, 134(9), 2108-2117. Wiley
ISSN: 0020-7136
Popis: Human papillomavirus (HPV) is a risk factor for the development of benign and malignant mucosal head and neck lesions. P16(INK4A) is often used as a surrogate marker for HPV-infection, although there is still controversy with respect its reliability. Our aim was to determine if P16(INK4A) overexpression can accurately predict both high-risk and low-risk-HPV-presence in (pre)malignant and benign head and neck lesions. P16(INK4A) immunohistochemistry was performed on paraffin-embedded tissue sections of 162 oropharyngeal squamous cell carcinomas (OPSCC), 14 tonsillar and 23 laryngeal dysplasias, and 20 tonsillar and 27 laryngeal papillomas. PCR, enzyme-immunoassay and FISH analysis were used to assess HPV-presence and type. Of the 162 OPSCC and 14 tonsillar dysplasias, 51 (31%) and 10 (71%) were HPV16-positive, respectively. All tonsillar papillomas were HPV-negative and four laryngeal dysplasias and 26 laryngeal papillomas were positive for HPV6 or -11. P16(INK4A) immunohistochemistry revealed a strong nuclear and cytoplasmic staining in 50 out of 51 HPV16-positive and 5 out of 111 HPV-negative OPSCC (p
Databáze: OpenAIRE
Externí odkaz: