The importance of including toxicity assays when screening plant extracts for antimalarial activity
| Autor: | Dyk, S., Griffiths, S., Robyn Van Zyl, Malan, S. F. |
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| Přispěvatelé: | 10065911 - Van Dyk, Sandra, 10199667 - Malan, Sarel Francois |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | African Journal of Biotechnology; Vol 8, No 20 (2009) Scopus-Elsevier |
| ISSN: | 1684-5315 |
| Popis: | Identifying plant extracts as sources of antimalarial compounds needs to be addressed as numerous studies screen extracts without the means of eliminating extracts that are merely cytotoxic. Fifty-nine organic solvent extracts from South African plants were screened for antiplasmodial activity using the [3H]-hypoxanthine incorporation assay against the chloroquine-resistant Plasmodium falciparum. Variable antiplasmodial activity and toxicity was observed. Extracts form Combretum erythrophyllum and Crinum bulbispermum, had IC50 values ≤ 1 μg/ml with the ethyl acetate extracts of C. bulbispermum roots and bulbs having values comparable to chloroquine (0.04 µg/ml). Nine extracts had toxicity indexes ≥ 100. Lycorine, isolated from C. bulbispermum was as active as chloroquine (IC50 of 0.03 µg/ml) and had a favourable security index. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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