Development of an elastography bench for histology and engineered tissue study-Preliminary results

Autor: Mathilde Bigot, Hugo Dorez, Celine Mandon, Christophe Marquette, Kevin Tse Ve Koon, Pauline M. Lefebvre, Denis Grenier, Hamza Raki, Christophe Marqué, Fabien Chauveau, Olivier Beuf, Simon Lambert
Přispěvatelé: RMN et optique : De la mesure au biomarqueur, Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé (CREATIS), Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-Hospices Civils de Lyon (HCL)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche et d'Application en Traitement de l'Image et du Signal (CREATIS), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ce travail a été réalisé grâce au soutien financier du LABEX PRIMES (ANR-11-LABX-0063) de l'Université de Lyon, dans le cadre du programme 'Investissements d'Avenir' (ANR-11-IDEX-0007) géré par l'Agence Nationale de la Recherche (ANR). PEPS CNRS 'Balanced'., Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Congrès National d'Imagerie du Vivant
Congrès National d'Imagerie du Vivant, Nov 2017, Paris, France
HAL
Popis: International audience; To study viscoelastic parameters of small ex vivo or engineered tissue samples, Magnetic Resonance Elastography (MRE) has been used during the last few years [1], [2]. It should enable a cautious sample-handling and be performed with a high spatial resolution. We describe here an elastography bench responding to those criteria. The setup is designed to image small samples (Fig.1). This requires an MRI coil with a high uniformity and filling factor to maximize the signal to noise ratio (SNR). Moreover the sample must be placed in the center of the coil and in contact with the mechanical transducer. Here, the sample holder is designed to slide into a 3D-printed support in which is included a Helmholtz coil tuned with two capacitor trimmers. Matching of the Helmholtz coil is done inductively using a circular coaxial coupling loop tuned at 200MHz. The sample holder is stopped at the center of the coil, guarantying the best RF uniformity and SNR. A cactus needle pierces the sample and is actuated by an MRI-compatible piezoelectric driver at 600Hz. Two samples were used to test the bench. The first one is made of polymerized fibrinogen (Sigma-F8630), which is used for engineered tissue. To limit motion of the sample, it was surrounded by a stiffer gel (DTM 133460). We also used a healthy rat brain embedded in agarose (Sigma A9414). The two embedding gels have well characterized mechanical properties and can serve as a reference.Acquisition of a FLASH and a MRE-compatible RARE sequence were made with a Bruker 4.7T scanner. Reconstruction of the viscoelastic parameters was done using an adapted method from Sinkus et al. [3].A voxel of 0.312x0.312x0.625mm3 with an SNR of 82.8 and 63.6 were obtained with the FLASH sequence, for the rat brain (Fig.2) and the phantom (Fig.3), respectively. Mean displacement amplitude for the RARE sequence was evaluated at 1.5µm and 5.6µm. Storage modulus representing elasticity was 1.8kPa and 1.4kPa. The gel surrounding the sample had an elasticity of 2.6kPa. Setup was easy to handle. It can be easily adapted to any MRI system. SNR is high and the induced mechanical displacement is enough to reconstruct viscoelastic parameters and highlight differences in elasticity between the commercial gel and the fibrinogen sample. Ex vivo results in brain are in agreement with literature [4].AcknowledgementThis work was supported by the LABEX PRIMES (ANR-XX-LABX-0063) of Université de Lyon, within the program "Investissements d'Avenir" (ANR-11-IDEX-0007) operated by the French National Research Agency (ANR).PEPS CNRS “Balanced”.References1-Boulet, J. Neurosci. Methods, 20112-Guertler, Proc. ISMRM, 20173-Sinkus, CR Mécanique, 20104-Millward, J. Neuroimmunol, 2014
Databáze: OpenAIRE
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