An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes

Autor: Bruyant, Flavienne, Babin, Marcel, Sciandra, A., Marie, Dominique, Genty, B., Claustre, Hervé, Blanchot, Jean, Bricaud, Annick, Rippka, R., Boulben, S., Louis, F., Partensky, Frédéric
Přispěvatelé: Laboratoire de Physique et Chimie Marines (LPCM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'océanographie de Villefranche (LOV), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Station biologique de Roscoff [Roscoff] (SBR), Laboratoire d'Ecophysiologie Végétale, Université Paris-Sud - Paris 11 (UP11), Institut Pasteur [Paris] (IP), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Zdroj: Journal of Applied Phycology
Journal of Applied Phycology, 2001, 13 (2), pp.135-142. ⟨10.1023/A:1011144310988⟩
Journal of Applied Phycology, Springer Verlag, 2001, 13 (2), pp.135-142. ⟨10.1023/A:1011144310988⟩
ISSN: 0921-8971
1573-5176
DOI: 10.1023/A:1011144310988⟩
Popis: International audience; A cyclostat was designed for growing the oceanic oxyphotobacterium Prochlorococcus PCC 9511. Culture of this organism, known to bedifficult to grow, was mastered for a large volume. Prochlorococcusgrew well and axenic conditions were maintained for up to 15 days. Wedesigned an illumination system allowing a smooth bell-shaped irradiancecurve reaching almost 1000 μmol quanta m-2 s-1 tobe obtained. Cell division was strongly synchronised under theseillumination conditions, which were close to those found at low latitude inthe upper layer of ocean. The described device is particularly well suited tomake experiments requiring up to 6 L per day of well synchronised,exponentially-growing Prochlorococcus culture.
Databáze: OpenAIRE
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