Determination of amino-acidic positions important for Ocimum basilicum geraniol synthase activity
| Autor: | Fischer, Marc, Meyer, Sophie, Claudel, Patricia, Steyer, Damien, Bergdoll, Marc, Hugueney, Philippe |
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| Přispěvatelé: | Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Advances in Bioscience and Biotechnology Advances in Bioscience and Biotechnology, 2013, 2013 (4), pp.242-249 Advances in Bioscience and Biotechnology, 2013, 4, pp.242-249 Advances in Bioscience and Biotechnology 4 (2013), 242-249. (2013) |
| Popis: | International audience; Terpenes are one of the largest and most diversified families of natural compounds. Although they have found numerous industrial applications, the molecular basis of their synthesis in plants has, until now, not been fully understood. Plant genomes have been shown to contain dozens of terpene synthase (TPS) genes, however knowledge of their amino-acidic protein sequence in not sufficient to predict which terpene(s) will be produced by a particular enzyme. In order to investigate the structural basis of a TPS specificity, we performed site directed mutations in the geraniol synthase from Ocimum basilicum. The results obtained suggest that a specific region on the catalytic site plays an important role in GPP transformation, either by stabilizing the GPP substrate on the catalytic site, or by enabling its transformation into a monoterpenol via an intermediate carbocation. |
| Databáze: | OpenAIRE |
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