| Autor: |
Jones, JA, Wilkins, DK, Smith, LJ, Dobson, CM |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Popis: |
The characterisation of non-native states of proteins is a key problem in studies of protein folding. Complete characterisation of these states requires a description of both local and global properties, including molecular dimensions. Here we present results from pulsed field gradient experiments designed to compare the effective hydrodynamic radii of a protein in native and non-native states. Measurements performed on lysozyme indicate that the effective hydrodynamic radius increases by 38±1% on unfolding in urea, a result completely consistent with a recent study by small-angle X-ray scattering. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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