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Enzimi su biološki katalizatori koji u posebno definiranim uvjetima ubrzavaju biokemijske reakcije u živim organizmima. Upravo u tu skupinu organskih makromolekula ubrajamo i proteaze. Prilikom izrade ovog rada, u eksperimentalnom dijelu su korištene tri komercijalne proteaze: Alkalaza, Novozyme 37071 i Novo-Pro D. Proteaze su korištene s ciljem hidrolize proteina u otpadu ljuski kozica. Provedbom te reakcije uklanjaju se proteini što predstavlja jedan od koraka koji bi omogućio izdvajanje preostalih korisnih spojeva koji u prirodi često dolaze zajedno. Ovaj način izdvajanja ima brojne prednosti u usporedbi s drugim poznatim metodama. Rezultati ukazuju na to da su enzimi bili vrlo efikasni u provođenju proteolitičke reakcije te se osobito iskazao biokatalizator Novo-Pro D. Enzymes are biological catalysts that accelerate biochemical reactions in organisms under specifically required conditions. Proteases also belong to the group of organic macromolecules called enzymes. Three commercial proteases were used in the experimental work in the laboratory: Alcalase, Novozyme 37071 and Novo-Pro D. The proteases were used to hydrolyze proteins found in shrimp waste. Protein hydrolysis removes the proteins, which is one of the steps that would allow the extraction of the remaining beneficial compounds that often occur together in nature. This type of extraction has many advantages over other methods that can be used. The results show that the proteases used for the experiments were very efficient in performing the proteolytic reaction, with Novo-Pro D being the most efficient. |
