PYL8 plays an important role for regulation of abscisic acid signaling in root
| Autor: | Antoni, Regina, González-Guzmán, Miguel, Peirats-Llobet, M., Pizzio, Gastón A., Fernández, M. Ángeles, De Winne, N, De Jaeger, G., Dietrich, D, Bennett, N. J. |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Abscisic acid (ABA) signaling plays a critical role to regulate root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work we have explored the role of Arabidopsis thaliana PYRABACTIN RESISTANCE1 (PYR1)/PYR1- LIKE (PYL)/ REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) ABA receptors for root ABA signaling. As a result, we discovered that PYL8 plays a non-redundant role for regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA-mediated root growth inhibition. This effect was due to lack of PYL8-mediated inhibition of several clade A PP2Cs since PYL8 interacted in vivo with at least five PP2Cs, namely HAB1, HAB2, ABI1, ABI2 and PP2CA/AHG3, as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABAhypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA-insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA-dependent inhibition of PP2Cs by PYR/PYLs is required for proper perception of a moisture gradient. |
| Databáze: | OpenAIRE |
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