| Popis: |
Human papillomaviruses (HPVs) belong to the family Papillomaviridae. Until now, 130 HPV types have been identified and fully sequenced. Approximately 40 types infect the anogenital tract and a few types are commonly found in anogenital cancer biopsy specimens. Detection and typing is important for the diagnosis of HPV associated diseases, notably cervical precancerous lesions and cervical cancer. The molecular methods used for HPV testing are based on the method of hybridization, DNA amplification (polymerase chain reaction – PCR) or both. In this study, 86 DNA samples, isolated from cervical scrapes with abnormal cytology (HSIL, high grade intraepithelial lesion) and determined as HPV positive by consensus PCR (MY09/11), were analyzed for the presence of specific HPV types by two commercially available line probe assays: LiPA from Innogenetics and Linear Array (LA) from Roche, that allow the detection of 25 and 37 HPV types, respectively. All 86 (100%) samples were successfully typed with Roche LA, while 79 (92%) samples were typed with LiPA. At least one HPV type was detected with both tests in 45 (52, 32%) cases, with LiPA detecting less HPV types as expected. Moreover, in 31 (36, 05%) cases, the results were identical between both tests, while in 3 cases (3, 49%) completely discordant. In this study group, we can conclude that Roche LA has an advantage over LiPA for HPV DNA genotyping because it allows the detection more HPV types in a single test with greater sensitivity. |
