Anti-inflammatory effects of autotaxin on microglial cells

Autor: Awada, R., GRES, S., Saulnier-Blache, J. S., Harry, G.Jean, Lefebvre d'Hellencourt, Christian
Přispěvatelé: Cyclotron Réunion Océan Indien (CYROI), Université de La Réunion (UR)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), National Institute of Environmental Health Sciences [Durham] (NIEHS-NIH), National Institutes of Health [Bethesda] (NIH), Diabète athérothrombose et thérapies Réunion Océan Indien (DéTROI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), NIEHS DIR, Région Réunion, Europe, Univ, Réunion, Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: FEBS Journal
FEBS Journal, Wiley, 2011, 36th FEBS Congress, Biochemistry for Tomorrow's Medicine, Lingotto Conference Center, Torino, Italy, June 25-30, 2011, 278, pp.266. ⟨10.1111/j.1742-4658.2011.08137.x⟩
FEBS Journal, 2011, 36th FEBS Congress, Biochemistry for Tomorrow's Medicine, Lingotto Conference Center, Torino, Italy, June 25-30, 2011, 278, pp.266. ⟨10.1111/j.1742-4658.2011.08137.x⟩
ISSN: 1742-464X
1742-4658
DOI: 10.1111/j.1742-4658.2011.08137.x⟩
Popis: International audience; Inflammation is essential in defence against infection or injury.However, overresponse can be detrimental, especially in immune-privileged organs such as the central nervous system, in whichmicroglia are the major source of inflammatory factors. Auto-taxin (ATX), a phospholipase D, converts lysophosphatidylcho-line into lysophosphatidic acid (LPA) and is upregulated inseveral CNS injuries. LPA, a pleiotropic immunomodulatory fac-tor, can induce multiple cellular processes, morphology, prolifera-tion, death and survival. Here, we investigated ATX effects onthe inflammatory response to two distinct inflammatory stimulitargeting microglia. Lipopolysacharide (LPS) mimics gram nega-tive infection, and trimethyltin (TMT), induces hippocampal neu-rodegeneration via the tumor necrosis factor (TNF) pathway.Murine BV2 microglia and stable transfected, overexpressingATX BV2 (BV2A+) were treated with LPS (1lg) and TMT(10lM). ATX, TNF, interleukin (IL)-6, IL-10 levels were exam-ined by qRT-PCR. LPA levels were determined. Microglial acti-vation markers (CD11b, CD14, B7.1, B7.2) were quantified byflow cytometry. ATX expression was significantly enhanced inLPS or TMT treated BV2. LPS-induced CD11b, CD14, and B7.1and B7.2 were reduced in BV2A+. TNF and IL-6 were inhibitedin LPS or TMT treated BV2A+ while IL-10 level was increased.In vivo, elevated ATX mRNA levels in the hippocampus weredemonstrated in CD1 mice 5 days following an acute ip injectionof TMT (2 mg/kg bwt). With the pattern and time course ofresponse, our results suggest an involvement of ATX in regulat-ing microglia activation and neuroinflammation.
Databáze: OpenAIRE
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