Diversity and seasonal dynamics of airborne archaea

Autor: Fröhlich-Nowoisky, J, Ruzene Nespoli, C., Pickersgrill, D.A., Galand, P.E., Müller-Germann, I., Nunes, T., Gomes Cardoso, J., Almeida, S.M., Pio, C., Andreae, M. O., Conrad, R., Pöschl, U., Després, V. R.
Přispěvatelé: Max Planck Institute for Chemistry (MPIC), Max-Planck-Gesellschaft, Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Universidade de Aveiro, University of Cape Verde, Universidade de Lisboa = University of Lisbon (ULISBOA), Max Planck Institute for Terrestrial Microbiology, Johannes Gutenberg - Universität Mainz (JGU), Universidade de Lisboa (ULISBOA)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Biogeosciences
Biogeosciences, 2014, 11 (21), pp.6067-6079. ⟨10.5194/bg-11-6067-2014⟩
Biogeosciences, European Geosciences Union, 2014, 11 (21), pp.6067-6079. ⟨10.5194/bg-11-6067-2014⟩
Biogeosciences, Vol 11, Iss 21, Pp 6067-6079 (2014)
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 1726-4170
1726-4189
DOI: 10.5194/bg-11-6067-2014⟩
Popis: Archaea are widespread and abundant in many terrestrial and aquatic environments, and are thus outside extreme environments, accounting for up to ~10% of the prokaryotes. Compared to bacteria and other microorganisms, however, very little is known about the abundance, diversity, and dispersal of archaea in the atmosphere. By means of DNA analysis and Sanger sequencing targeting the 16S rRNA (435 sequences) and amoA genes in samples of air particulate matter collected over 1 year at a continental sampling site in Germany, we obtained first insights into the seasonal dynamics of airborne archaea. The detected archaea were identified as Thaumarchaeota or Euryarchaeota, with soil Thaumarchaeota (group I.1b) being present in all samples. The normalized species richness of Thaumarchaeota correlated positively with relative humidity and negatively with temperature. This together with an increase in bare agricultural soil surfaces may explain the diversity peaks observed in fall and winter. The detected Euryarchaeota were mainly predicted methanogens with a low relative frequency of occurrence. A slight increase in their frequency during spring may be linked to fertilization processes in the surrounding agricultural fields. Comparison with samples from the Cape Verde islands (72 sequences) and from other coastal and continental sites indicates that the proportions of Euryarchaeota are enhanced in coastal air, which is consistent with their suggested abundance in marine surface waters. We conclude that air transport may play an important role in the dispersal of archaea, including assumed ammonia-oxidizing Thaumarchaeota and methanogens.
Databáze: OpenAIRE
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