Expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori
| Autor: | Joosten, V., Gouka, R.J., Hondel, C.A.M.J.J. van den, Verrips, C.T., Lokman, B.C. |
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| Přispěvatelé: | TNO Kwaliteit van Leven TNO Voeding |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Heavy-chain antibody fragments
Expression vector Molecular Sequence Data Immunoglobulin Variable Region Gene Expression Protein degradation Antibody production Antibodies Lama glama Degradation mRNA levels Transformation Genetic vectors Functions Animals Extracellular space Aspergillus expression Amino Acid Sequence Cloning Molecular Biology Antibody Sequence Homology Amino Acid Messenger RNA Protein Fungi Molecular cloning Proteins Culture medium Nonhuman Recombinant Proteins Vanadium compounds Aspergillus Aspergillus awamori RNA Protein expression Immunoglobulin Heavy Chains Nucleotide sequence Camelids New World Biotechnology Cloning |
| Zdroj: | Applied Microbiology and Biotechnology, 4, 66, 384-392 |
| Popis: | We report the expression and production of llama variable heavy-chain antibody fragments (VHHs) by Aspergillus awamori. Fragments encoding VHHs were cloned in a suitable Aspergillus expression vector and transformants secreting VHH fragments were analysed for integrated gene copy-numbers, mRNA levels and protein production. Functional V HHs were detected in the culture medium, indicating the feasibility of producing this type of protein in a fungal expression system. Secreted V HHs were subjected to (extracellular) degradation, which could be partially prevented by the addition of BSA to the culture medium. © Springer-Verlag 2004. |
| Databáze: | OpenAIRE |
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