Calcium homeostasis and modulation of nociceptive synaptic transmission
| Autor: | Sojka, David |
|---|---|
| Přispěvatelé: | Paleček, Jiří, Žurmanová, Jitka, Krůšek, Jan |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
VÁPNÍK
PAIN NMDA ENDOPLASMATICKÉ RETICULUM NOCICEPCE DORSAL HORN INTRACELLULAR STORES CALCIUM VNITRNÍ ZDROJE FLUO-4 CICR METABOTROPIC RECEPTORS MGLUR BOLEST CALCIUM-INDUCED CALCIUM-RELEASE GLIA GLIE CENTRÁLNÍ SENSITIZACE CALCIUM IMAGING KAPSAICIN SPINAL CORD CAPSAICIN ER METABOTROPNÍ RECEPTORY AMPA CENTRAL SENSITIZATION ENDOPLASMIC RETICULUM MÍCHA ZADNÍ ROH MÍŠNÍ GABA |
| Popis: | This study was designed to improve our knowledge regarding mechanisms of nociceptive signaling at spinal cord level. One of the forms of spinal cord synaptic transmission modulation is central sensitization, a manifestation of synaptic plasticity at spinal cord level, which was found to be present at many chronic pain syndromes. This study deals mainly with a development of calcium imaging technique with a final goal to study mechanisms of central sensitization in vitro on population of dorsal horn neurons. We have analyzed synaptically evoked intracellular Ca changes as a result of dorsal root stimulation in a superficial dorsal horn area in spinal cord slices and found two types of Ca responses: one synchronized with electrical stimulation and a second one, delayed response due to Ca release from internal stores. The delayed Ca release was not previously shown to be present in these neurons and it was not dependent on activation of ionotropic glutamatergic receptors, suggesting involvement of metabotropic receptor pathway. The presence of this delayed type of Ca response could have a significant role in the induction of some types of chronic pain syndromes, since intracellular calcium increase is thought to be a key trigger point in spinal cord neurons sensitization. An important role in neuronal calcium... |
| Databáze: | OpenAIRE |
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