Stem Cell Reports Resource Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells

Autor: Santos, Renata, Vadodaria, Krishna, Jaeger, Baptiste, Mei, Arianna, Lefcochilos-Fogelquist, Sabrina, Mendes, Ana P.D., Erikson, Galina, Shokhirev, Maxim, Randolph-Moore, Lynne, Fredlender, Callie, Dave, Sonia, Oefner, Ruth, Fitzpatrick, Conor, Pena, Monique, Barron, Jerika, Ku, Manching, Denli, Ahmet, Kerman, Bilal, Charnay, Patrick, Kelsoe, John, Marchetto, Maria, Gage, Fred
Přispěvatelé: Institut de psychiatrie et neurosciences de Paris (IPNP - U1266 Inserm - Paris Descartes), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris)-École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Psychiatry, University of California [San Diego] (UC San Diego), University of California-University of California, The Salk Institute for Biological Studies, Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS Paris
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Current Stem Cell Reports
Current Stem Cell Reports, Springer, 2017, 8 (6), pp.1757-1769. ⟨10.1016/j.stemcr.2017.05.011⟩
ISSN: 2198-7866
DOI: 10.1016/j.stemcr.2017.05.011⟩
Popis: International audience; Astrocyte dysfunction and neuroinflammation are detrimental features in multiple pathologies of the CNS. Therefore, the development of methods that produce functional human astrocytes represents an advance in the study of neurological diseases. Here we report an efficient method for inflammation-responsive astrocyte generation from induced pluripotent stem cells (iPSCs) and embryonic stem cells. This protocol uses an intermediate glial progenitor stage and generates functional astrocytes that show levels of glutamate uptake and calcium activation comparable with those observed in human primary astrocytes. Stimulation of stem cell-derived astrocytes with interleukin-1b or tumor necrosis factor a elicits a strong and rapid pro-inflammatory response. RNA-sequencing transcriptome profiling confirmed that similar gene expression changes occurred in iPSC-derived and primary astrocytes upon stimulation with interleukin-1b. This protocol represents an important tool for modeling in-a-dish neuro-logical diseases with an inflammatory component, allowing for the investigation of the role of diseased astrocytes in neuronal degeneration.
Databáze: OpenAIRE
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