Direct and indirect interactions between cannabinoid CB1 receptor and group II metabotropic glutamate receptor signalling in layer V pyramidal neurons from the rat prefrontal cortex

Autor: Barbara, J.G., Auclair, N., Roisin, M.P., Otani, S., Valjent, E., Caboche, J., Soubrie, P., Crepel, F.
Přispěvatelé: Neurobiologie des processus adaptatifs (NPA), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Signalisation Cellulaire et Parasites, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5), Centre de Recherche, SANOFI Recherche, This work was financially supported in part by grants to W.J.A.J.H. from the European Community Research Fund (EU-TMR Network contract number CT2000-00085) and the Dutch Cancer Society (Koningin Wilhelmina Fonds grant number KUN 98-1810)., Neurobiologie des processus adaptatifs ( NPA ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP]-Université Paris Descartes - Paris 5 ( UPD5 ), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP]-Université Paris Descartes - Paris 5 (UPD5)
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Zdroj: European Journal of Neuroscience
European Journal of Neuroscience, Wiley, 2003, 17, pp.981-990. ⟨10.1046/j.1460-9568.2003.02533.x⟩
European Journal of Neuroscience, Wiley, 2003, 17, pp.981-990. 〈10.1046/j.1460-9568.2003.02533.x〉
European Journal of Neuroscience, 2003, 17, pp.981-990. ⟨10.1046/j.1460-9568.2003.02533.x⟩
ISSN: 0953-816X
1460-9568
DOI: 10.1046/j.1460-9568.2003.02533.x⟩
Popis: At proximal synapses from layer V pyramidal neurons from the rat prefrontal cortex, activation of group II metabotropic glutamate receptors (group II mGlu) by (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl) glycine (DCG IV) induced a long-lasting depression of excitatory postsynaptic currents. Paired-pulse experiments suggested that the depression was expressed presynaptically. Activation of type 1 cannabinoid receptors (CB1) by WIN 55,212-2 occluded the DCG IV-induced depression in a mutually occlusive manner. At the postsynaptic level, WIN 55,212-2 and DCG IV were also occlusive for the activation of extracellular signal-regulated kinase. The postsynaptic localization of active extracellular signal-regulated kinase was confirmed by immunocytochemistry after activation of CB1 receptors. However, phosphorylation of extracellular signal-regulated kinase in layer V pyramidal neurons was dependent on the activation of N-methyl-d-aspartate receptors, consequently to a release of glutamate in the local network. Group II mGlu were also shown to be involved in long-term changes in synaptic plasticity induced by high frequency stimulations. The group II mGlu antagonist (RS)-alpha-methylserine-O-phosphate monophenyl ester (MSOPPE) favoured long-term depression. However, no interaction was found between MSOPPE, WIN 55,212-2 and the CB1 receptor antagonist SR 141716A on the modulation of long-term depression or long-term potentiation and the effects of these drugs were rather additive. We suggest that CB1 receptor and group II mGlu signalling may interact through a presynaptic mechanism in the induction of a DCG IV-induced depression. Postsynaptically, an indirect interaction occurs for activation of extracellular signal-regulated kinase. However, none of these interactions seem to play a role in synaptic plasticities induced with high frequency stimulations.
Databáze: OpenAIRE
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