Dni1p and Dni2p: two claudin-like proteins required for cell fusion

Autor: Curto, María Ángeles, Sharifmoghadam, M. R., Clemente-Ramos, José Ángel, Valdivieso, María Henar
Rok vydání: 2015
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Resumen del póster presentado al Young Life Scientists’ Symposium: "Cell cycle: the challenge to coordinate genome stability and morphogenesis", celebrado en Salamanca (España) el 4 de diciembre del 2015.
Cell-cell fusion is a essential process that occurs in many cell types during development and underlies sexual reproduction. During mating in Schizosaccharomyces pombe, cell differentiate into shmoos which can fuse in order to give rise to a zygote, so this event is an excellent model to get insight into plasma membrane fusion mechanisms. The implication od Dni1 and Dni2 proteins in this process has been addressed. Deletion of any or both of these proteins causes a failure in cell fusion at medium-high temeprature because of an abnormal organization of plasma membrane and cell wall at the region of cell-cell contact. However, mutants are able to fuse at low temperature, which suggests taht Dni1p and Dni2p facilitate fusion under conditions of elevated membrane fluidity. Dni1p and Dni2p have four transmembrane domains and the structure properties of claudins. Structure-function analyses demonstrate that in Dni2p, but not in Dni1p, these functional properties are required for function. Additionally, both proteins have small-XXX-small domains play a role in functionality. While Dni2p is localized at the endoplasmic reticulum before cell fusion, Dni1p is only detected at the plasma membrane (PM). At the moment of cell fusion both proteins co-localize at as PM microdomain at the point of cell-cell contact. Both proteins must be present in the cell for the proper localization of each other. It seems that Dni1p and Dni2p form a complex where each one could exert a specific funtion; while Dni1 is the functional component, Dni2 is required to establish/maintain the microdomain where Dni1p needs to locate to play its role. In addition to this, endocytosis and correct actin organization contribute to the proper accumulation of these proteins in this microdomain.
Databáze: OpenAIRE
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