Optimization of pressurized liquid extraction and evaluation of antioxidant, anti-inflammatory and anti-Alzheimer activities in vitro of Ammodaucus Leucotrichus
| Autor: | Abderrezag, Norelhouda, Louaer, Ouahida, Meniai, Abdeslam-Hassen, Mendiola, J. A. |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministry of Higher Education and Scientific Research (Algeria) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Trabajo presentado a la 1st European Sample Preparation e-Conference, celebrada del 11 al 12 de marzo de 2021. The extraction of high added value products is an issue of great interest and importance especially when investigated in new plants belonging to arid and semi-arid zones, which have been not enough studied. Within this objective, we select a spontaneous endemic plant; Ammodaucus leucotrichus, native to Saharan and sub-Saharan countries of north and tropical Africa. It is used In traditional medicine to treat stomach diseases, allergy symptoms, diabetes and cardiac diseases and also in foods as condiment or flavouring agent. Pressurized liquid extraction PLE is one of the most widely used technique in extracting bioactive compounds from natural sources; PLE (also known as accelerated solvent extraction, ASE) can employ a wide range of solvents at high pressure and temperature above their boiling point. It is an environmentally friendly, quick and efficient technology; in addition, the amount of solvent used is very small, so the concentration stage can be avoided. PLE has been used for the first time to extract phenolic compounds from Ammodaucus leucotrichus, two environmental friendly solvents, water and ethanol, at different temperatures (40, 110 and 180 ºC) for 20 min at 10 MPa were applied in order to optimize extraction yield and total phenolic compound (TPC). Moreover, antioxidant activity by Free radical scavenging (DPPH) and anti-neurodegenerative by anticholinesterase (AChE) and anti-inflammatory by inhibition of lipoxygenase (LOX) were evaluated in vitro. The results showed that Ammodaucus leucotrichus has significant antioxidant, anti-Alzheimer and anti-inflammatory activities. The optimal conditions were achieved at 180 ºC using water, which was a better extraction solvent than ethanol. Extraction yield was positively influenced by temperature in a range of 15.55 to 44.45 %. Furthermore, antioxidant activity, inhibition of acetylcholinesterase and inhibition of lipoxygenase showed the same behavior with the following EC50 values: 85.51 ug/ml, 55.60 ug/mL, 39.37 ug/mL for DPPH, AChE and LOX, respectively. To summarize, the new PLE procedure can be considered a green and efficient method for extraction of phenolics from Ammodaucus leucotrichus, making it possible to produce extracts with attractive antioxidant, anti-Alzheimer and anti-inflammatory for future pharmaceutical and food applications. This work has been funded by the project AGL2017-89417-R (Spanish Ministry of Science and Innovation). N. Abderrezag are grateful to the Salah Bobnider Constantine 3 university, Constantine, Algeria for the fellowship. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|