GENE EXPRESSION ANALYSIS OF AUTOPHAGY GENES IN POLYCYTHEMIA VERA PATIENTS
| Autor: | Smetana, Nicolai Frederik |
|---|---|
| Přispěvatelé: | Novak Nakir, Ivana, Korać Prlić, Jelena, Pecotić, Renata, Omerović, Jasminka |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Popis: | Objectives: There is an increasing interest in the correlation between autophagy/mitophagy processes in myeloproliferative neoplasms like polycythemia vera. Especially the Department of Immunology and Medical Genetics at the University of Split, School of Medicine conducts intensive research in this field. The aim of the study was to analyze the expression of autophagy/mitophagy related genes in PV patients in order to find out, if a difference is observable compared to a control group. Materials and methods: We used blood samples from eight PV patients to test the expression of seven autophagy/mitophagy related genes via qPCR. We then differentiated into JAK2 positive and negative patients and analyzed accordingly. Results: After performing qPCR and data analysis, we could see that some genes in JAK2 positive PV patients were expressed more and some less than in the leukemia control. The results were not statistically significant. Conclusion: The decreased expression of some autophagy and mitophagy related genes in PV patients could be an indicator for the importance of auto- and mitophagy in PV. Especially Nix and Bnip3, which both showed a decreased expression, might be indicative, because they already have been shown to play a crucial role in cancer and heart disease. Further studies are needed to evaluate the significance of this relationship. Cilj istraživanja: Postoji povećan interes u povezanosti autofagije/mitofagije u mijeloproliferativnim neoplazmama poput policitemije vere. Posebice na Odjelu za imunologiju i medicinsku genetiku na Medicinskom fakultetu, Sveučilišta u Splitu provode opsežna istraživanja u tom području. Cilj ovog istraživanja bio je analizirati pojavnost gena povezanih s autofagijom/mitofagijom u pacijenata oboljelih od PV kako bi otkrili postoji li zamjetna razlika prema kontrolnoj skupini. Materijali i metode: Koristili smo uzorke krvi od 8 pacijenata oboljelih od PV kako bismo pomoću qPCR testirali pojavnost sedam gena povezanih s autofagijom/mitofagijom. Zatim smo pacijente podijelili u JAK2 pozitivne i negativne i sukladno tome napravili analizu. Rezultati: Nakon provedbe qPCR i analize podataka, primjetili smo da su neki geni u JAK2 pozitivnih pacijenata s PV izraženiji, a neki manje izraženi, nego u kontrolnoj skupini s leukemijom. Dobiveni rezultati nisu bili statistički značajni. Zaključak: Smanjena ekspresija nekih gena povezanih s autofagijom i mitofagijom u pacijenata s PV može biti pokazatelj važnosti autofagije i mitofagije u PV. Pogotovo Nix i Bnip3, koji su oba bili manje izraženi, jer se već otprije pokazalo kako imaju važnu ulogu u nastanku karcinoma i bolesti srca. Daljnje studije su potrebne za procjenu važnosti ove povezanosti. |
| Databáze: | OpenAIRE |
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