A validated system for ligation-free USER™ -based assembly of expression vectors for mammalian cell engineering
| Autor: | Anne Mathilde Lund, Helene Faustrup Kildegaard, Bjarne Gram Hansen, Dorte Koefoed Holm, Mikael Rørdam Andersen, Uffe Mortensen |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Zdroj: | Lund, A M, Kildegaard, H F, Hansen, B G, Holm, D K, Andersen, M R & Mortensen, U H 2013, ' A validated system for ligation-free USER™-based assembly of expression vectors for mammalian cell engineering ' 23rd ESACT meeting, Lille, France, 23/06/2013-26/06/2013, . Technical University of Denmark Orbit |
| Popis: | The development in the field of mammalian cell factories require fast and high-throughput methods, this means a high need for simpler and more efficient cloning techniques. For optimization of protein expression by genetic engineering and for allowing metabolic engineering in mammalian cells, a new versatile expression vector system was developed. This vector system applies the ligation-free uracilexcision cloning technique to construct mammalian expression vectors of multiple parts and with maximum flexibility. |
| Databáze: | OpenAIRE |
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