Long term effects of an early exposure to PAHs on zebrafish behavioural responses

Autor: Vignet, Caroline, Lemenach, Karyn, Péan, Samuel, Lyphout, Laura, Leguay, Didier, Budzinski, Hélène, Begout, Marie Laure, Cousin, Xavier
Přispěvatelé: Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Physico -& Toxico Chimie des systèmes naturels (LPTC), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Place Gaby Coll - BP 7, Station de l'Houmeau, MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire d'Écotoxicologie, Laboratoire Ressources Halieutiques, Place Gaby Coll, BP 7, Place Gaby Coll, BP 7, Département Dynamiques de l'Environnement Côtier, Halieutique Gascogne Sud [La Rochelle] (IFREMER HGS), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Fisheries Society of the British Isles (FSBI), Université Sciences et Technologies - Bordeaux 1, Fisheries Society of the British Isles (FSBI). GBR., Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ressources Halieutiques (LRH), Society of Environmental Toxicology and Chemistry (SETAC), Vignet, Caroline, Laboratoire des Ressources Halieutiques (IFREMER), Département Systèmes Sous-Marins - IFREMER, Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: 23. SETAC Europe Annual Meeting
23. SETAC Europe Annual Meeting, May 2013, Glasgow, United Kingdom
FSBI 2012. Annual International Symposium
FSBI 2012. Annual International Symposium, Fisheries Society of the British Isles (FSBI), Jul 2012, Norwich, United Kingdom
FSBI 2012. Annual International Symposium, Fisheries Society of the British Isles (FSBI). GBR., Jul 2012, Norwich, United Kingdom
6th SETAC World Congress/SETAC Europe 22 nd Annual Meeting
6th SETAC World Congress/SETAC Europe 22 nd Annual Meeting, May 2012, Berlin, Germany. pp.355, 2012, Abstract book-6th SETAC World Congress/SETAC Europe 22nd Annual Meeting
23. Annual Meeting SETAC Europe
23. Annual Meeting SETAC Europe, May 2013, Glasgow, United Kingdom
Popis: International audience; Polycyclic aromatic hydrocarbons (PAH) emission in the environment is constantly increasing with human activity. The goal of this study was to assess long term consequences of fish early exposure to PAH on behaviour. Eggs were collected from AB zebrafish strain and were placed at 4 hours post fertilization (hpf) until 96 hpf in a 3 cm diameter Petri dish containing 3 g reference sediment either plain (control) or spiked with a mixture of Benzo[a]pyrene, Pyrene and Phenanthrene at environmental concentrations based on values measured in the Seine Estuary (France), a highly polluted site. Thereafter, larvae were transferred in clean water and raised until adulthood. Behavioural tests such as locomotion during 24-hrs, sudden dark change challenge and T-maze exploration were performed at adults’ stage (F0) and sudden dark change on larvae produced by F0 adults. In adults, during night or dark period, contaminated fish were significantly less active than control fish. A similar observation was made for contaminated F0 in T-maze safe area. Contaminated F1 larvae were more active when light was on than control and less active when the light was off. This study indicates that contamination of zebrafish using environmentally relevant concentration during the very first stage of development with a PAH mixture of 3 molecules induced late behavioural effects measured at the adult stage. Further, effect was transmitted to the next generation and behavioural responses of F1 larvae were different to that of F1 control larvae. Consequences on fish abilities (e.g. predator escape, food search, courtship) will be discussed.
Databáze: OpenAIRE
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