Reação de acessos de Lycopersicon spp. a um isolado de Potato Virus Y (PVYº) de tomateiro

Autor: Palazzo, Silvia Regina Luz, Colariccio, Addolorata, Melo, Arlete Marchi Tavares de
Jazyk: portugalština
Rok vydání: 2008
Předmět:
Zdroj: Bragantia v.67 n.2 2008
Bragantia
Instituto Agronômico de Campinas (IAC)
instacron:IAC
Bragantia, Volume: 67, Issue: 2, Pages: 391-399, Published: 2008
Popis: Amostras de tomateiro (Lycopersicon esculentum Mill) ‘Alambra’ coletadas nas regiões produtoras de Elias Fausto, Monte-Mor e Mogi-Guaçú (SP), com sintomas de amarelecimento foliar generalizado semelhante aqueles causados por vírus foram submetidas à identificação do agente causal, por testes biológicos de transmissão mecânica, pela determinação do círculo de hospedeiras, empregando-se plantas indicadoras e diferenciadoras pertencentes às famílias Chenopodiaceae e Solanaceae pela identificação sorológica por PTA-ELISA do Potato virus Y (PVY), Pepper yellow mosaic virus (PepYMV), Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV) e DAS-ELISA com antissoros policlonais para as espécies Tomato spot wilt vírus (TSWV), Tomato chlorotic spot virus (TCSV), Groundnut ringspot virus (GRSV), Chrysanthemum stem necrosis virus (CSNV) e anticorpos monoclonais para as estirpes do PVY comum (PVYº), PVY necrótico (PVY N) e PVY clorótico (PVY C). Das amostras coletadas 19 reagiram positivamente, com o PVY em PTA-ELISA e PVYºem DAS-ELISA. Plantas de Chenopodium amaranticolor reagiram com sintoma local e plantas de Nicotiana glutinosa, N. tabacum ‘WB’, N. sylvestris, N.debneyi, N. tabacum ‘Sansun’ reagiram com sintomas de mosaico sistêmico; tomateiros ‘Alambra’ manifestaram sintomas de mosaico-amarelo. Plantas de Datura stramonium, D. metel e C. annuum ‘Magda’ não foram infectadas. A ausência de sintomas em C.annuum ‘Magda’, identificou a presença do PVY patotipo 1 (PVYº1), nas 19 amostras. Como foi identificada a mesma espécie de vírus nas amostras, optou-se pela inoculação do isolado de tomate ‘Alambra’ de Elias Fausto nos dezenove acessos de Lycopersicon spp. do Banco Ativo de Germoplasma (BAG)- IAC. O delineamento dos experimentos foi inteiramente casualizado. A reação dos acessos foi avaliada pela manifestação dos sintomas, pelos resultados positivos ou negativos após testes de retro-inoculação e PTA-ELISA, pela análise do χ2 utilizando-se a proporção de plantas sintomáticas e assintomáticas, com resultados positivos ou que não manifestaram sintomas e reagiram negativamente, em PTA-ELISA, constituindo, portanto fontes potenciais de genes de resistência para o PVY em tomate. Samples of tomato (Lycopersicon esculentum Mill) ‘Alambra’ were collected in commercial fields of Elias Fausto, Monte-Mor and Mogi-Guaçu, SP. The plants with virus-like symptoms, especially yellow mosaic were submitted, to biological and serological identification tests. For biological tests, the host range was determined by indicator and differential plants in the family Chenopodiaceae and Solanaceae. The serological identification was made by PTA-ELISA with polyclonal antiserum against Tospovirus, Tobamovirus, Potyvirus and Cucumovirus and by DAS-ELISA, by using monoclonal antibodies against PVY common strain (PVYº), PVY chlorotic strain (PVY C) and PVY necrotic strain (PVY N). All the tested samples showed positive reaction in PTA-ELISA with antiserum against PVY and negative reaction with the antiserum against the remaining viruses. In DAS-ELISA, positive reaction occurred with PVY common strain. Among the host plants, Chenopodium amaranticolor reacted with local lesions, while N. glutinosa and N. tabacum ‘WB’ showed systemic mosaic and the ‘Alambra’ reacted with yellow mosaic, while Datura stramonium, D. metel and C. annuum ‘Magda’ were not infected. The reaction on the differential host C. annuum ‘Magda’ permitted to identify the PVY pathotype 1 in all the 19 samples. To evaluate the reaction of the 19 accesses of Lycopersicon spp. from the IAC active bank of germoplasm, one of the samples of tomato ‘Alambra’ from Elias Fausto was inoculated on tomato accesses and also, the variety ‘Alambra’ was used as a positive control. The experiments delineation was randomized and the plant reaction was evaluated by presence and type of symptoms, and also by positive and negative results after back inoculation and serological tests (PTA-ELISA), and by analysis of χ2 using the ratios between symptomatic and asymptomatic plants with positive or negative results in ELISA. It was verified that most evaluated accesses were tolerant to PVYº-1, but the wild species L. peruvianum B, LA-371-2 and radiated 1C had a great number of asymptomatic plants with negative results by ELISA, constituting potential sources of genes of resistance against PVY in tomatoes.
Databáze: OpenAIRE
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