Local estrogen metabolism in epithelial ovarian cancer suggests novel targets for therapy
| Autor: | Ren, Xia, Wu, Xuan, Hillier, Stephen G., Fegan, K. Scott, Critchley, Hilary O.D., Mason, J. Ian, Sarvi, Sana, Harlow, Christopher R. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
endocrine system
endocrine system diseases Endocrinology Diabetes and Metabolism Clinical Biochemistry Cell Biology Epithelial ovarian cancer Biochemistry Estrogen Estrogen sulfotransferase female genital diseases and pregnancy complications Estrogen Steroid sulfatase Estrogen sulfotransferase Epithelial ovarian cancer Ovarian surface epithelium Ovarian surface epithelium Endocrinology Steroid sulfatase Molecular Medicine Molecular Biology |
| Zdroj: | Ren, X, Wu, X, Hillier, S G, Fegan, K S, Critchley, H O D, Mason, J I, Sarvi, S & Harlow, C R 2015, ' Local estrogen metabolism in epithelial ovarian cancer suggests novel targets for therapy ', Journal of Steroid Biochemistry and Molecular Biology, vol. 150, pp. 54-63 . https://doi.org/10.1016/j.jsbmb.2015.03.010 |
| DOI: | 10.1016/j.jsbmb.2015.03.010 |
| Popis: | Epithelial ovarian cancer (EOC) accounts for about 90% of malignant ovarian tumors, and estrogen is often implicated in disease progression. We therefore compared the potential for gating of estrogen action via pre-receptor metabolism in normal human ovarian surface epithelium (OSE), EOC and selected EOC cell lines (SKOV3 and PEO1). Steroid sulphatase (STS), estrogen sulfotransferase (EST), 17β-hydroxysteroid dehydrogenases 2 (17BHSD2) and 5 (17BHSD5) mRNAs, proteins and enzymatic activities were all detectable in primary cell cultures of OSE and EOC, whereas aromatase and 17BHSD1 expression was negligible. qRT-PCR assay on total mRNA revealed significantly higher EST mRNA expression in OSE compared to EOC (P |
| Databáze: | OpenAIRE |
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